Translational_Unit

Part:BBa_K4773017:Design

Designed by: Yan Pan   Group: iGEM23_UESTC-China   (2023-10-02)


RBS-HAO


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 288
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

RBS (BBa_B0034) was chosen because it has a high translation initiation rate and is compatible with most bacterial hosts.


Source

HAO is a hydroxylamine oxidoreductase from Nitrosomonas europaea.

References